Table of Contents
What is centrifuged urine?
After a urine (pee) sample is collected, it’s put into a centrifuge — a special machine that separates the liquid in the urine from any solid components that may be present, such as blood cells, mineral crystals, or microorganisms. Any solid materials are then viewed under a microscope.
Where does the liquid portion of urine come from?
Urine is a liquid byproduct of the body secreted by the kidneys through a process called urination and excreted through the urethra. The normal chemical composition of urine is mainly water content, but it also includes nitrogenous molecules, such as urea, as well as creatinine and other metabolic waste components.
Why is urine centrifuged for a microscopic examination?
Because it is necessary to observe bacteria, fungi and leucocytes in the examination of urinary tract infection, centrifugal force in the urinary sediment preparation is able to set higher speed and longer time than the normal urinary sediment preparation.
What is the importance of urine centrifugation before testing for the presence of protein?
Protein. Dipstick screening for protein is done on whole urine, but semi-quantitative tests for urine protein should be performed on the supernatant of centrifuged urine since the cells suspended in normal urine can produce a falsely high estimation of protein.
What does it mean to spin urine?
Before it is examined under a microscope, technicians spin the urine in a centrifuge in order to concentrate the solid particles and make them easy to examine. Microscopic examination might reveal the following: (3) White blood cells may indicate infection or inflammation.
Does urine need to be centrifuged?
Urine should be spun at a slow speed. Most centrifuges have a radial arm of ~14.5 cm and should be spun at ~1,500 rpm (with a range of 1,000−2,000 rpm) for ~5 minutes (with a range of 3–5 minutes, longer time for slower speeds).
What is cessation of urine flow?
Anuria is when the kidneys stop producing urine. The condition is usually the result of disease or damage to the kidneys.
What is the correct order that waste is eliminated out of the body from the urinary system?
Urine exits the bladder and the body through the urethra. The kidneys, ureters, bladder, and urethra make up the urinary tract, the pathway through which urine flows and is eliminated from the body.
How long do you spin urine in a centrifuge?
How do you spin down urine?
- Collect a fresh urine sample (5 – 10 mL).
- Spin the sample in a centrifuge at around 1500 rpm (revolutions per minute) for 3-5 minutes (make sure you balance your sample with a test tube with similar volume)
- You should see a pellet at the bottom of the tube.
What is SSA urine test?
The sulfosalicylic acid (SSA) test measures urine turbidity when a small amount of aspirin is added to the urine specimen. A finding of +2 to +4 suggests bacteriuria.
Is a clear urine always normal explain your answer?
If a person experiences clear urine, they do not usually need to take any further action. Clear urine is a sign of good hydration and a healthy urinary tract. However, if they consistently notice clear urine and also have extreme or unusual thirst, it is best to speak to a doctor.